Phylogenetic composition and properties of bacteria coexisting with the fungus, de Boer W., van der Wal A. “Interactions between saprotrophic basidiomycetes and bacteria,’ in. Sterile water was used to moisten the wood and a sterile swab was rolled along the wood’s surface. Micrococcus kristinae 4. 1a). Image 5 and 6 are from the Kraken app. To thrive in this community it would be helpful to have mechanisms to process radical oxygen species. Genus planococcus. The results did not meet these thresholds, and this is the reason that the BaseSpace indentified taxanomy of Lysinibacillus sphaericus was rejected. Planococcaceae, a family within the order Bacillales, embraces 14 genera: Bhargavaea, Caryophanon, Chryseomicrobium, Filibacter, Jeotgalibacillus, Kurthia, Paenisporosarcina, Planococcus, Planomicrobium, Rummeliibacillus, Solibacillus, Sporosarcina, Ureibacillus, and Viridibacillus.Members of the family are Gram-variable, spore forming or nonspore forming, and motile … If any microbes grew from the firewood it would mean that they were able to withstand temperatures below -50 degrees Celsius and be psycrophiles, if any microbes grew in my wood stove they would have had to be thermophiles. Each of those colonies represented a microbe that had the physiological capability of surviving those conditions, and I was interested in observing them in the lab. The gram stain was performed to assess the thickness of the peptidoglycan wall. The quadratic streak was used 4 times to ensure a pure culture. nov. isolated from cyanobacterial mat samples collected from ponds in Antarctica. PAMC21323 is a gram (+), motile, psy-chrotrophic bacteria, which can grow over a broad temperature range (5–40 °C). Using Illumina’s BaseSpace dashboard to handle the data, the isolate’s genome was analyzed and compared to an existing database to search for similar sequences of DNA. To conclusively Identify the taxonomy of this isolated microbe, it would be advisable to repeat DNA isolation and genomic analysis that was preformed in this project, and to additionally perform other genotypic analyses like, DNA-DNA hybridization and 16s rRNA analysis and fatty acid identity. Gram stain. Halotolerant will grow in nutrient agar An obligate aerobe, M. luteus is found in soil, dust, water and air, and as part of the normal microbiota of the mammalian skin. To control growth rates the plates were grown in incubators for 2-4 days at 37 degrees Celsius then moved to a refrigerator to ensure colonies did not grow large enough to touch. Pigments may be produced by some species; ranging from cream, pale yellow to light pink. Inst. Overnight grown pathogenic bacterial strains of 1 × 10 6 CFU/mL were treated with known concentration of biosurfactants and incubated at … Yellow colonies that’s size increases with time, a smooth round margin, elevated dome. the following information is not yet verified. Micrococcaceae are not a coherent group. Planococcus faecalis sp. My isolate was not just aerobic but a facultative aerobe, which is inconsistent with two articles that state the genus is strictly aerobic5. The purity of the isolate was confirmed with the microscopic observation of a mono-culture. Planomicrobium okeanokoites was reclassified from Flavobacterium okeanokoites. Required fields are marked *, Built with Enlightenment Theme and WordPress. The negative results and lack of growth in the API 20e, MAC, and EMB are all consistent with the microbe’s gram-positive cell wall. The Inhibition Zones exceed the antibiotic specific threshold diameter for susceptibility see table 2 for details. The next app that was used was the Kraken Metagenomics, which provided taxonomic assignment. Microscopically, it is a cocci-shaped bacterium measuring 0.5 to 0.7 μmin diameter (Fig. Micrococcus luteus is a Gram-positive, to Gram-variable, nonmotile, coccus, tetrad-arranging, pigmented, saprotrophic bacterium that belongs to the family Micrococcaceae. The results of the SPAdes Genome Assembler were above required values; # contigs >=1000 bp should be in the hundreds, total length should be at least a million bp and the longest contig should be at least 100000 bp. The literature on the Planococcus species like donghaensis, kocurii, halocryophilus and antarticus often notes how closely related the species are and how further analysis like G-C content, fatty acid strains, DNA-DNA hybridization etc1,2 are needed to differentiate the species. BLAST had a different database and could possibly have the genome of the isolates species if base space did not. Some of the notable differences between Micrococcus and Staphylococcus are: One representative of each from gram stain positive and gram stain negative bacteria was selected to further assess the MIC. Planococcus sp. The general features of Planococcus sp. BaseSpace has many apps that provide different information about the genomic data. The morphological characteristics that are consistent with the isolate and across the genus are: gram-positive membranes, cocci cell shape, colonies are yellow orange in color, catalase positive and an aerobic oxygen class5. Planococcus citri, commonly known as the citrus mealybug, is a species of mealybugs native to Asia.It has been introduced to the rest of the world, including Europe, the Americas, and Oceania, as an agricultural pest.It is associated with citrus, but it attacks a … There are some qualities that are consistent between my sample and P. donghaensis, they are both, gram-positive, aerobic, coccus and oxidase positive 1. nov. M. Engelhardt , K. Daly , R. Swannell , I. Abstract. Of this information, only the number of contigs that were greater than 1000 base pairs long [# contigs (>=1000bp)], The total length of the assembled genome, the longest contig and the guanine and cytosine percentage in the genome (GC%) were observed. The cell walls of Gram positive bacteria differ structurally from the cell … Staphylococcus in Gram Stain Both of these organisms are normal flora of skin and mucous membranes of man and animals. Melissococcus ein neues System der Bakterien. haemolysans, Lactococcus Leuconostoc, The genus Planococcus was originally proposed by Migula [1] to accommodate Gram-stain-positive, aerobic and motile cocci of the family Planococcaceae in the phylum Firmicutes. Stomatococcus The Microbes transfer to the agar slant was successful: Image 1, and was successfully re-cultured from the slant when necessary. It was identified in 1894, and was only approved in 19806. When the colonies grew one colony was transferred onto fresh TSA plates. The Prokka Genome Annotation app identified 64 tRNAs, 0 rRNAs, 1 CRISPR, and 3739 CDS. I sampled two places, a piece of wood from my fire wood stack (which is exposed to the extreme cold temperatures of Alaskan winters) and the ashes from my stove. The gram stain was performed to assess the thickness of the peptidoglycan wall. An orange pigmented bacterium, S1, was isolated from a cyanobacterial mat sample collected in the vicinity of Schirmacher Oasis, Maitri, the Indian station, inAntarctica. Considering the environment in which it was found, there were a few expected results: such as the microbe’s oxygen class and catalase test results. P. donghaensis may have had the ability to deal with these temperatures in its genome, but it is not certain since it’s environment doesn’t select for such characteristics. 1. Bacterium Planococcus halocryophilus that is able to thrive at minus 15 degrees Celsius offers clues about microbial life on both Mars and Enceladus. A series of patented solutions C1-4 were added following the protocol to purify and remove inhibitors and proteins until all that remained was a pure solution of DNA. Euzeby, J. P., & Parte, A. C. (2017, April 1). Member of the genus Staphylococcus are associated with clinical infections whereas that of Micrococcus are rarely involved in infection. , while the Arb. Cell lysis was accomplished through adding sodium dodecyl sulfate (SDS), which is a surfactant that chemically weakened the cell walls, and the use of PowerBeads and the centrifuge to mechanically break the cell walls. (2016). PAMC21323 are shown in Table 1. Planococcus sp. The APP provides an extensive list of regions that were specifically identified as the above elements. The sample was collected from a dry piece of wood. MAC plates are also selective for Gram-negative microbes and differentiates between lactose fermenters (agar turns pink) and non-lactose-fermenters (agar turns colorless). Micrococcus nishinomiyaensis 7. Image 6 To 20 Classification Results by Taxonomic Level from BaseSpace. Your email address will not be published. Karlsruhe 1:235-238, p 1781 Genera related Using the protocol of lab 6 the physiological characteristics were tested. Several other species had similar query cover, including Planococcus antarticus: Image 7. A gram-positive (gram-variable), beaded organism with delicate branching was recovered from the sputum of a 20-year-old patient with leukemia. BLAST suggested Genus and species with associated query cover percentage and identity percentage. The species classification with the highest query cover is poorly documented in literature. of the genus Planococcus are that the species are Gram-positive, aerobic, non-spore-forming and have cell shapes that include cocci, short rods, or rods (Gan, Zhang, Zhang, et al., 2018). Colonies are round and yellow in color. It is urease and catalase positive. The cells were measured to be 1 micrometer in diameter. The genotypic test results for this microbe were vague, the results of BaseSpace had a low number of reads that could be classified and of those reads there was little consensus. Micrococcus lylae 6. The gram-stain was repeated three times before a successful stain was accomplished. The second most likely species suggested by was P. antarticus, at least this species shares a similar environment. The firewood pile was slowly decomposing, and it is likely that the microbe that was isolated from it was a part of the community that was utilizing the wood as an energy source. Craters may form around the colonies on some media. nov., a carotenoid-producing species isolated from stools of Antarctic penguins. 5. While under the microscope it was observed that the cells could be aligned linearly, in pairs or quads but not in a longer strep chain. 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